The Cadillac and Chevrolet maker said in April it would close its production operations in Ecuador and neighboring Colombia.
General Motors' OBB plant in Quito accounts for 51% of car production in Ecuador.
The factory's 320 workers fear for their livelihoods. Ecuador's unemployment in the first quarter of the year was 4.1%, according to official figures.
"I have mixed feelings - I'm welding my last truck for General Motors in Ecuador," said welding team leader Antonio Oramas, who joined the plant in 2004. "It will affect us a lot. Not all of us will have the same opportunity for a new job."
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